West Indian spinner Akeal Hosein and Batter Jyd Goolie failed to travel to the UK for the next matches against England and Ireland because of visa issues.
Both remain in Trinidad, having failed to gain visas after the UK registration requirements were updated to the citizens of Trinidad and Tobago on April 23.
Hosein, 32, according to the global T20 bowling ranking, was scheduled to play in the first T20 against England at Chester-le-Street on Friday.
Updated requirements mean that both players had to participate in compulsory personal commitments in Trinidad before receiving permission to travel, but Hosein could not do so over time, as he was playing in the Super League of Pakistan.
Cricketary West Indies (CWI) expects to resolve the matter, but it is unlikely to be done in time from Hosein, whose passport remains with the authorities, appearing against England.
The second T20 against England is on Sunday in Bristol and the third on Tuesday in Southampton.
Goolie, 28, was a late addition to the three -match T20 Series team against Ireland, as a replacement for Roston Chase after he was appointed West Indian test captain last month.
Chase is returning to the Caribbean to prepare for a series of tests against Australia later this month, taking -from the T20s against Ireland, which are being played at Bready Cricket Club in Northern Ireland. Hosein is also on the team to play Ireland.
“Once the updated visa requirements were announced, CWI started the necessary registration processes. This was only possible after Akeal returned to Trinidad and after confirming the JYD selection to the Ireland Squad,” said CWI CWI director Miles Bascombe.
A CWI statement added: “CWI made formal efforts to accelerate the visa process for Akeal and requested a resignation from the visa for JYD.
“However, these processes are governed by the UK immigration authorities and remain out of CWI control.
“CWI is still optimistic that the matter will be resolved in the coming days, allowing Hosein to join the squad for the remaining matches.”
The home office of the UK was approached to comment.
England Spinner Shoaib Bashir lost a test in India last year After problems, gaining a visa.
